In the first game out on Tuesday morning, Michael Smyth (Royal Troon) lost on the final green to Angus Carrick (Douglas Park), by a single hole.
Steven Stamper (Turnberry) soon exited the championship also, going down 3 & 2 against Greig Kennedy (Hazlehead).
Fortunes turned in the next two ties involving Ayrshire players, Sandy Buchanan (Prestwick St Nicholas) winning by 6 & 5 against Gavin Sutherland (Reay) to set up a second round encounter with Andrew davidson (Charleton), whilst Jim Johnston (Ayr Belleisle) beat mark Hislop (Glenbervie) at the first extra hole to progress to a second round meeting with David Mitchell (Leven Thistle).
The last Ayrshire player in first round action was current Ayrshire Strokeplay Champion Greg Telfer (Prestwick St Cuthbert), but he suffered an early exit from the event with a 5 & 3 defeat by Rob Paterson (Kilspindie).
Three Ayrshire players were in second round action late on Tuesday but there was no joy for the local players with all three losing their respective matches.
David Wilson (Troon Welbeck) went down by 3 & 2 to Steven Buchan (Royal Aberdeen), Mark McMillan (Turnberry) lost by 2 & 1 to Dan Lawrence (Ballumbie Castle) whilst Craig Telfer (Prestwick St Cuthbert lost out to Alastair Thurlow (Murrayfield) by the same margin.
The five Ayrshire players still in the championship are scheduled to complete their second round ties on Wednesday morning before all the third round matches, which are due for completion by close of play on Wednesday.