Both teams fielded very strong teams in the match which was played in fine conditions.
The Ayrshire side was led by Scotland internationalist and African Amateur Strokeplay Champion Euan Walker (Kilmarnock Barassie) who got the county side off to a winning start with a 2&1 win over Stuart Robin.
Toimmy McInally (Louodun) and John Shanks (Irvine) added wins by the same margin over Scott Brown & Steven Wallace respectively.
In the anchor tie, Robbie Muir (Kilmarnock Barassie) beat Steven Gillies by 3&1 whilst Craig Hamilton overcame Graeme Gimson by 4&3 in their tie to give Ayrshire a 5 - 0 advantage.
The only reverse suffered by Ayrshire in the match came when Michael Smyth (Royal Troon) lost out to Craig Telfer by 2 holes.
Match details (Prestwick St Cuthbert names first)
Stuart Robin lost to Euan Walker (Kilmarnock Barassie) by 2&1
Scott Brown lost to Tommy McInally (Louodun) by 2&1
Steven Wallace lost to John Shanks (Irvine) by 2&1
Craig Telfer beat Michael Smyth (Royal Troon) by 2 holes
Graeme Gimson lost to Craig Hamilton (Ballochmyle) by 4&3
Steven Gillies lost to Robbie Muir (Kilmarnock Barassie) by 3&1
PRESTWICK ST CUTHBERT 1 AYRSHIRE 5